Publications

2013

R.N. Maamari, J.D. Keenan, D.A. Fletcher, T.P. Margolis, "A mobile phone-based retinal camera for portable wide field imaging", Br J Ophthalmol. 2013 Dec 16. doi: 10.1136/bjophthalmol-2013-303797. [Epub ahead of print] [Abstract]

M.C. Good, M.D. Vahey, A. Skandarajah, D.A. Fletcher, R. Heald, "Cytoplasmic volume modulates spindle size during embryogenesis" , Science. 2013 Nov 15;342(6160):856-60. doi: 10.1126/science.1243147. [Abstract]

B.L. Ricca, G. Venugopalan, D.A. Fletcher, "To pull or be pulled: parsing the multiple modes of mechanotransduction", Curr Opin Cell Biol. 2013 Oct;25(5):558-64. doi: 10.1016/j.ceb.2013.06.002. Epub 2013 Jul 2. [Abstract]

S. Barreto, C.H. Clausen, C.M. Perrault, D.A. Fletcher, D. Lacroix, "A multi-structural single cell model of force-induced interactions of cytoskeletal components", Biomaterials. 2013 Aug;34(26):6119-26. doi: 10.1016/j.biomaterials.2013.04.022. Epub 2013 May 21. [Abstract]

D.G. Brownfield, G. Venugopalan, A. Lo, H. Mori, K. Tanner, D.A. Fletcher, M.J. Bissell, "Patterened Collagen Fibers Orient Branching Mammary Epithelium through Distinct Signaling Modules", Curr Biol. 2013 Apr 22;23(8):703-9. doi: 10.1016/j.cub.2013.03.032. Epub 2013 Apr 4. [Article]

A. Tapley, N. Switz, C. Reber, J.L. Davis, C. Miller, J.B. Matovu, W. Worodria, L. Huang, D.A. Fletcher, A. Cattamanchi, "Mobile Digital Fluorescence Microscopy for Diagnosis of Tuberculosis", J Clin Microbiol. 2013 Jun;51(6):1774-8. doi: 10.1128/JCM.03432-12. Epub 2013 Apr 3. [Article]

2012

A. Diz-Muñoz, D.A. Fletcher, O.D. Weiner, "Use the force: membrane tension as an organizer of cell shape and motility", Trends Cell Biol. 2013 Feb;23(2):47-53. doi: 10.1016/j.tcb.2012.09.006. Epub 2012 Nov 2. Review. [Article]

J. Chang, P. Arbeláez, N. Switz, C. Reber, A. Tapley, J.L. Davis, A. Cattamanchi, D.A. Fletcher, J. Malik, "Automated tuberculosis diagnosis using fluorescence images from a mobile microscope", Med Image Comput Comput Assist Interv. 2012;15(Pt 3):345-52. [Article]

J.C. Stachowiak, E.M. Schmid , C.J. Ryan, H.S. Ann, D.Y. Sasaki, M.B. Sherman, P.L. Geissler, D.A. Fletcher, C.C. Hayden, "Membrane bending by protein-protein crowding", Nat Cell Biol. 2012 Sep;14(9):944-9. doi: 10.1038/ncb2561. Epub 2012 Aug 19. [Article]

V.I. Risca, E.B. Wang, O. Chaudhuri, J.J. Chia, P.L. Geissler, D.A. Fletcher, “Actin filament curvature biases branching direction”, Proceedings of the National Academy of Sciences USA, vol 109 issue 8, 2913-2918, February 2012. Epub January 2012. [Article]

A. Crow, K.D. Webster, E. Hohlfeld, W.P. Ng, P. Geissler, D.A. Fletcher, “Contractile equilibriation of single cells to step changes in extracellular stiffness”, Biophysical Journal, vol 102 issue 3, 443-451, February 2012. [Article]

M. Tsai, A. Kita, J. Leach, R. Rounsevell, J.N. Huang, J. Moake, R.E. Ware, D.A. Fletcher, W.A. Lam, “In vitro modeling of the microvascular occlusion and thrombosis that occur in hematologic diseases using microfluidic technology”, The Journal of Clinical Investigation, vol 122 issue 1, 408-418, January 2012. Epub December 2011. [Article]

2011

A. Kita, Y. Sakuri, D.R. Myers, R. Rounsevell, J.N. Huang, T.J. Seok, K. Yu, M.C. Wu, D.A. Fletcher, W.A. Lam, "Microenvironmental geometry guides platelet adhesion and spreading: A quantitative analysis at the single cell level", PLoS ONE, vol 6 issue 10, e26437, October 2011. Epub ahead of print. [Article]

D.L. Richmond, E.M. Schmid, S. Martens, J.C. Stachowiak, N. Liska, D.A. Fletcher, "Forming giant vesicles with controlled membrane composition, asymmetry, and contents", PNAS, vol 108 issue 23, 9431-9436, June 2011. Epub ahead of print, May 2011. [Article]

K. Webster, A. Crow, D.A. Fletcher, "An AFM-based stiffness clamp for dynamic control of rigidity", PLoS ONE, vol. 6 issue 3, e17807, March 2011. [Article]

R. Novak, Y. Zeng, J. Shuga, G. Venugopalan, D.A. Fletcher, M.T. Smith, R.A. Mathies, "Single-Cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions", Angewandte Chemie International Edition, vol. 50 issue 2, 390-395, January 2011. [Article]

W.A. Lam, O.Chaudhuri, A. Crow, K.D. Webster, T. Li, A. Kita, J. Huang, D.A. Fletcher, "Mechanics and contraction dynamics of single platelets and implications for clot stiffening", Nature Materials, vol. 10 No 1, 61-66, January 2011. [Article]

2010

D.A. Fletcher, "On force and function", Molecular Biology of the Cell, vol. 21 issue 22, 3795-6, 15 November 2010. [Abstract]

A. Pasqua, L. Maibaum, G. Oster, D.A. Fletcher, "Large-scale simulations of fluctuating biological membranes", Journal of Chemical Physics, vol. 132 issue 15 article 154107, April 2010. [Abstract]

G. Bao, R.D. Kamm, W. Thomas, W. Hwang, D.A. Fletcher, A.J. Grodzinsky, C. Zhu, M.R. Mofrad, "Molecular Biomechanics: The Molecular Basis of How Forces Regulate Cellular Function", Molecular & Cellular Biomechanics, vol. 3 No. 2, 91-105, March 2010. [Article]

D.A. Fletcher and R.D. Mullins, "Cell mechanics and the cytoskeleton", Nature, vol. 463, 485-492, 28 January, 2010. [Article]

2009

A.P. Liu and D.A. Fletcher, "Biology under construction: in vitro reconstitution of cellular function", Nature Reviews Molecular Cell Biology vol. 10, 644-650, September, 2009. [Article]

D.N. Breslauer, R.N. Maamari, N.A. Switz, W.A. Lam, D.A. Fletcher, "Mobile phone based clinical microscopy for global health applications", PLoS ONE 4(7): e6320, July 2009. [Article]

J.C. Stachowiak, D.L. Richmond, T.H. Li, F. Brochard-Wyart and D.A. Fletcher, "Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation", Lab on a Chip, vol. 9 No. 14, 2003-2009, June 2009. [Article]

D.A. Fletcher and P.L. Geissler, "Active biological materials", Annual Review of Physical Chemistry, vol. 60, 469-486, 2009. [Article]

J.C. Stachowiak, T.H. Li, A. Arora, S. Mitragotri, D.A. Fletcher, "Dynamic control of needle-free jet injection", J. Control Release, vol. 135(2), 104-112, April 2009. [Article]

O. Chaudhuri, S.H. Parekh, W.A. Lam, D.A. Fletcher, "Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells", Nature Methods, vol. 6(5), 383-387, April 2009. [Article]

T.H. Li, J.C. Stachowiak, D.A. Fletcher, "Mixing solutions in inkjet formed vesicles", Methods in Enzymol, vol. 465, 75-94, 2009. [Article]

2008

M.J. Rosenbluth, A. Crow, J.W. Shaevitz, D.A. Fletcher, "Slow Stress Propagation in Adherent Cells" Biophysical J vol. 95, 6052-6059, December 2008.[Article]

S.C. Hsiao, A.K. Crow, W.A. Lam, C.R. Bertozzi, D.A. Fletcher, M.B. Francis, "DNA-coated AFM cantilevers for the investigation of cell adhesion and the patterning of live cells" Angew Chem Int Ed Engl vol. 47, 8473-8477, 2008. [Article]

A.P. Liu, D.L. Richmond, L. Maibaum, S. Pronk, P.L. Geissler, D.A. Fletcher, "Membrane-induced bundling of actin filaments", Nature Physics vol. 4, 789-793, October 2008. [Article]

S. Pronk, P.L. Geissler, D.A. Fletcher, "Limits of filipodium stability" Phys Rev Lett vol. 100, 258102, June 2008. [Article]

M.J. Rosenbluth, W.A. Lam, D.A. Fletcher, "Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry" Lab Chip vol. 8, 1062-1070, July 2008. [Article]

J.W. Shaevitz, D.A. Fletcher, "Curvature and torsion in growing actin networks" Phys Biol vol. 5, 26006, June 2008. [Article]

W.A. Lam, M.J. Rosenbluth, D.A. Fletcher, "Increased leukaemia cell stiffness is associated with symptoms of leucostasis in paediatric acute lymphoblastic leukaemia" Br J Haematol vol. 142, 480-501, June 2008. [Article]

J.C. Stachowiak, D.L. Richmond, T.H. Li, A.P. Liu, S.H. Parekh, D.A. Fletcher, "Unilamellar vesicle formation and encapsulation by microfluidic jetting" PNAS, vol. 105, 4697-702, March 2008. [Article]

W.R. Hansen, D.A. Fletcher, "Tonic shock induces detachment of Giardia lamblia" PLoS Negl Trop Dis. 2008 Feb;2(2):e169. [Article]

2007

J.C. Stachowiak, M.G. von Muhlen, T.H. Li, L. Jalilian, S.H. Parekh, D.A. Fletcher, "Piezoelectric control of needle-free transdermal drug delivery" J Control Release vol. 124, 88-97, December 2007. [Abstract]

J.W. Shaevitz, D.A. Fletcher, "Load fluctuations drive actin network growth" PNAS vol. 104, 15688-92, October 2007. [Abstract]

J.W. Shaevitz, D.A. Fletcher, "Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point spread function" JOSA A vol. 24, 2622-7, September 2007. [Abstract]

C.I. Lacayo, Z. Pincus, M.M. van Duijn, C.A. Wilson, D.A. Fletcher, F.B. Gertler, A. Mogilner, J.A. Theriot, "Emergence of large-scale cell morphology and movement from local actin filament growth dynamics" PLoS Biol. vol. 5, e233, September 2007. [Abstract]

J.L. Choy, S.H. Parekh, O. Chaudhuri, A.P. Liu, C. Bustamante, M.J. Footer, J.A. Theriot, D.A. Fletcher, "Differential force microscope for long time-scale biophysical measurements" Review of Scientific Insturments, vol. 78, April 2007. [Abstract]

W.A. Lam, M.J. Rosenbluth, and D.A. Fletcher, "Chemotherapy exposure increases leukemia cell stiffness" Blood, vol. 109, 3505-8, April 2007. [Abstract]

Arora A, Hakim I, Baxter J, Rathnasingham R, Srinivasan R, Fletcher DA, Mitragotri S., "Needle-free delivery of macromolecules across the skin by nanoliter-volume pulsed microjets" PNAS, vol. 104, 4255-60, March 2007. [Abstract]

O.Chaudhuri, S.H. Parekh, and D.A. Fletcher, "Reversible stress softening of actin networks" Nature, vol 445, 295-298, January 2007. [Abstract]

2006

A.P. Liu and D.A.Fletcher, "Actin polymerization serves as a membrane domain switch in model lipid bilayers" Biophysical Journal, vol. 91 4064-4070, December 2006. [Abstract]

C.M. Nelson, M.M. Van Duijn, J.L. Inman, D.A. Fletcher, and M.J. Bissell, "Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic Cultures" Science, 13 October 2006. [Abstract]

A.P. Arkin and D.A. Fletcher, "Fast, cheap, and somewhat in control" Genome Biology, 30 August 2006. [Abstract]

W.R. Hansen, O Tulyathan, S.C. Dawson, W.Z. Cande, and D. A. Fletcher, "Giardia lamblia Attachment Force is Insensitive to Surface Treatments" Eukaryotic Cell, April 2006. [Abstract]

M.J. Rosenbluth, W.A. Lam, and D.A. Fletcher, "Force microscopy of nonadherent cells: A comparison of leukemia cell deformability", Biophysical Journal, vol. 90 no. 8, 2994-3003, April 2006. [Abstract]

2005

S.H. Parekh, O. Chaudhuri, J. Theriot, and D.A. Fletcher, "Loading history determines velocity of actin network growth", Nature Cell Biology, vol. 7, no. 12, 1219-1223, 2005. [Abstract]

J.W. Shaevitz, J.Y. Lee, D.A. Fletcher, “Spiroplasma Swim by a Processive Change in Body Helicity”, Cell, vol. 122, no. 6, 941-935, 2005. [Abstract]

A.P. Liu, D.A.Fletcher, “Photopatterning of actin filament structures”, Nano Letters, vol. 5, no. 4, 625-628, 2005. [Abstract]

2004

D. A. Fletcher and J. A. Theriot, “An introduction to cell motility for the physical scientist”, Physical Biology, vol. 1, no. 1, T1-T10, 2004. [Abstract]

S.S. Justice, C. Hung, J.A. Theriot, D.A. Fletcher, G.G. Anderson, M.J. Footer, and S.J. Hultgren, “Differentiation and developmental pathways of uropathogenic E. coli in urinary tract pathogenesis”, PNAS, vol. 101, no. 5, pp. 1333-1338, 2004. [Abstract]

2003

D.A. Fletcher, G.S. Kino, and K.E. Goodson, “Thermal microscopy with a microfabricated solid immersion lens”, Microscale Thermophysical Engineering, vol. 7, no. 4, pp. 267-273, 2003. (cover image)

P.A. Giardini, D.A. Fletcher, J.A. Theriot, “Compression forces generated by actin comet tails on lipid vesicles”, PNAS, vol. 100, no. 11, pp. 6493-6498, 2003. (Science Editor’s Choice, June 6, 2003) [Abstract]

2002

D.A. Fletcher, D.V. Palanker, P. Huie, J. Miller, M.F. Marmor, and M.S. Blumenkranz, “Intravascular drug delivery with the pulsed liquid microjet,” Archives of Ophthalmology, vol. 120, pp. 1206-1208, 2002. [Abstract]

K.B. Crozier, D.A. Fletcher, G.S. Kino, and C.F. Quate, “Micromachined silicon nitride solid immersion lens”, JMEMS, vol. 11, pp. 470-478, 2002.

2001

D.A. Fletcher, K.B. Crozier, K.W. Guarini, S.C. Minne, G.S. Kino, C.F. Quate, and K. E. Goodson, “Microfabricated silicon solid immersion lens,” JMEMS, vol. 10, pp. 450-459, 2001.

D.A. Fletcher, K.B. Crozier, C.F. Quate, G.S. Kino, K.E. Goodson, D. Simanovskii, and D.V. Palanker, “Refraction contrast imaging with a scanning microlens”, Applied Physics Letters, vol. 78, pp. 3589-3591, 2001.

D.A. Fletcher, K.E. Goodson, and G.S. Kino, “Focusing in microlenses close to a wavelength in diameter,” Optics Letters, vol. 26, pp. 399-401, 2001. [Abstract]

D. A. Fletcher and D.V. Palanker, “Pulsed liquid microjet for microsurgery,” Applied Physics Letters, vol. 78, pp. 1933-1935, 2001. Nature News, April 3, 2001. New Scientist News, April 25, 2001.

2000

D.A. Fletcher, K.B. Crozier, G.S. Kino, C.F. Quate, K.E. Goodson, D. Simanovskii, and D.V. Palanker, “Near-field infrared imaging with a microfabricated solid immersion lens,” Applied Physics Letters, vol. 77, pp. 2109-2111, 2000.

1999

T. Sulchek, S.C. Minne, J.D. Adams, D.A. Fletcher, A. Atalar, C.F. Quate, and D.M. Adderton, “Dual integrated actuators for extended range high speed atomic force microscopy,” Applied Physics Letters, vol. 75, pp. 1637-1639, 1999.