Bead-based RNA Detection with Cas13a: Multi-scale Design and Validation
Biorxiv (2026); DOI: 10.64898/2026.07.27.739218
Bhuiya AM, Agarwal S, Ng CF, Ott M, Son S, Fletcher DA

LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs
Biorxiv (2026); DOI: 10.64898/2026.02.02.700737
Ng CF*, Krishnamurthy D*, Dextre A, Chorlay A, Ott M, Fletcher DA

Programmable kinetic barcoding for multiplexed RNA detection with Cas13a
Nature Biomedical Engineering (2026); DOI: 10.1038/s41551-026-01642-6
Son S, Lyden A, Ng CF, Dextre A, Shu J, Stephens SI, Fozouni P, Knott GJ, Smock DCJ, Liu TY, Boehm D, Simoneau C, Kumar GR, Doudna JA, Ott M, Fletcher DA

NTDscope: A multi-contrast portable microscope for disease diagnosis
PLOS Global Public Health (2026); DOI: 10.1371/journal.pgph.0005937
Díaz de León Derby M, Moussa ZL, Ng CF, Bhuiya AM, Cabrera JP, Banik D, Delahunt CB, Keller MD, Le Ny ALM, Garcia-Villena J, Dacal E, Bermejo-Peláez D, Cuadrado D, Luengo-Oroz M, Djeunga HCN, Kamgno J, Yemeli LD, Pahl V, Davi SD, Manego RZ, Ramharter M, Bogoch II, Coulibaly JT, Khatun A, Kabir M, Noor Z, Haque R, Switz NA, Friedman DH, D’Ambrosio MV, Fletcher DA

SurFlex microscopy: Measuring flexibility of surface-tethered biomolecules
PNAS (2025); DOI: 10.1073/pnas.2508828122
Chorlay A, Agarwal S, Blackmon L, Dextre A, Fletcher DA

Multi-contrast machine learning improves schistosomiasis diagnostic performance
PLOS Neglected Tropical Diseases (2025); DOI: 10.1371/journal.pntd.0012879
Díaz de León Derby M, Delahunt CB, Spencer E, Coulibaly JT, Silue KD, Bogoch II, Le Ny ALM, Fletcher DA

Point-of-Care Sample Preparation and Automated Quantitative Detection of Schistosoma haematobium Using Mobile Phone Microscopy
American Journal of Tropical Medicine and Hygiene (2022); DOI: 10.4269/ajtmh.21-1071
Armstrong M, Harris AR, D’Ambrosio MV, Coulibaly JT, Essien-Baidoo S, Ephraim RKD, Andrews JR, Bogoch II, Fletcher DA

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
Cell (2021); DOI: 10.1016/j.cell.2020.12.001
Fozouni P*, Son S*, Díaz de León Derby M*, Knott GJ, Gray CN, D’Ambrosio MV, Zhao C, Switz NA, Kumar GR, Stephens SI, Boehm D, Tsou CL, Shu J, Bhuiya A, Armstrong M, Harris A, Osterloh JM, Meyer-Franke A, Langelier C, Pollard KS, Crawford ED, Puschnik AS, Phelps M, Kistler A, DeRisi JL, Doudna JA, Fletcher DA, Ott M

A Smartphone-Based Tool for Rapid, Portable, and Automated Wide-Field Retinal Imaging
Translational Vision Science & Technology (2018); DOI: 10.1167/tvst.7.5.21
Kim TN, Myers F, Reber C, Loury PJ, Loumou P, Webster D, Echanique C, Li P, Davila JR, Maamari RN, Switz NA, Keenan J, Woodward MA, Paulus YM, Margolis T, Fletcher DA

In Southern Nigeria Loa loa Blood Microfilaria Density is Very Low Even in Areas with High Prevalence of Loiasis: Results of a Survey Using the New LoaScope Technology
American Journal of Tropical Medicine and Hygiene (2018); DOI: 10.4269/ajtmh.18-0163
Emukah E, Rakers LJ, Kahansim B, Miri ES, Nwoke BEB, Griswold E, Saka Y, Anagbogu I, Davies E, Ityonzughul C, D’Ambrosio M, Bakalar M, Fletcher DA, Nutman T, Kamgno J, Richards FO

A Test-and-Not-Treat Strategy for Onchocerciasis in Loa loa–Endemic Areas
New England Journal of Medicine (2017); DOI: 10.1056/NEJMoa1705026
Kamgno J, Pion SD, Chesnais CB, Bakalar MH, D’Ambrosio MV, Mackenzie CD, Nana-Djeunga HC, Gounoue-Kamkumo R, Njitchouang GR, Nwane P, Tchatchueng-Mbouga JB, Wanji S, Stolk WA, Fletcher DA, Klion AD, Nutman TB, and Boussinesq M

Accuracy of Mobile Phone and Handheld Light Microscopy for the Diagnosis of Schistosomiasis and Intestinal Protozoa Infections in Côte d’Ivoire
PLoS Neglected Tropical Diseases (2016); DOI: 10.1371/journal.pntd.0004768
Coulibaly JT, Ouattara M, D’Ambrosio MV, Fletcher DA, Keiser J, Utzinger J, N’Goran EK, Andrews JR, Bogoch II

Evaluation of mobile digital light-emitting diode fluorescence microscopy in Hanoi, Viet Nam
International Journal of Tuberculosis and Lung Disease (2015); DOI: 10.5588/ijtld.15.0018
Chaisson LH, Reber C, Phan H, Switz N, Nilsson LM, Myers F, Nhung NV, Luu L, Pham T, Vu C, Nguyen H, Nguyen A, Dinh T, Nahid P, Fletcher DA, Cattamanchi A

Assessment of a Smartphone Otoscope Device for the Diagnosis and Management of Otitis Media
Clinical Pediatrics (2015); DOI: 10.1177/0009922815593909
Rappaport KM, McCracken CC, Beniflah J, Little WK, Fletcher DA, Lam WA, Shane AL.

Multi-Contrast Imaging and Digital Refocusing on a Mobile Microscope with a Domed LED Array
PLoS One. 10(5), eCollection (2015)
Phillips ZF, D’Ambrosio MV, Tian L, Rulison JJ, Patel HS, Sadras N, Gande AV, Switz NA, Fletcher DA, Waller L.

Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope
Science Translational Medicine (2015); DOI: 10.1126/scitranslmed.aaa3480
D’Ambrosio MV, Bakalar M, Bennuru S, Reber C, Skandarajah A, Nilsson L, Switz N, Kamgno J, Pion S, Boussinesq M, Nutman TB, Fletcher DA

Diagnosis of Schistosoma haematobium Infection with a Mobile Phone-Mounted Foldscope and a Reversed-Lens CellScope in Ghana
American Journal of Tropical Medicine and Hygiene (2015); DOI: 10.4269/ajtmh.14-0741
Ephraim RK, Duah E, Cybulski JS, Prakash M, D’Ambrosio MV, Fletcher DA, Keiser J, Andrews JR, Bogoch II

A lateral electrophoretic flow diagnostic assay
Lab Chip (2015); DOI: 10.1039/c4lc01370k
Lin R, Skandarajah A, Gerver RE, Neira HD, Fletcher DA, Herr AE

Novel telemedicine device for diagnosis of corneal abrasions and ulcers in resource-poor settings
JAMA Ophthalmol. 132, 894–895 (2014)
R.N. Maamari, S. Ausayakhun, T.P. Margolis, D.A. Fletcher, J.D. Keenan

Low-cost mobile phone microscopy with a reversed mobile phone camera lens
PLOS ONE (2014); DOI: 10.1371/journal.pone.0095330
Switz NA, D’Ambrosio MV, Fletcher DA

Quantitative imaging with a mobile phone microscope
PLOS ONE (2014); DOI: 10.1371/journal.pone.0096906
Skandarajah A, Reber CD, Switz NA, Fletcher DA

Lipid bilayer vesicle generation using microfluidic jetting
J Vis Exp. 84, e51510 (2014). doi:10.3791/51510
C.W. Coyne, K. Patel, J. Heureaux, J. Stachowiak, D.A. Fletcher, A.P. Liu

A mobile phone-based retinal camera for portable wide field imaging
Br J Ophthalmol. 98, 438–441 (2014)
R.N. Maamari, J.D. Keenan, D.A. Fletcher, T.P. Margolis

Mobile Digital Fluorescence Microscopy for Diagnosis of Tuberculosis
J. Clin. Microbiol. 51, 1774–1778 (2013)
A. Tapley, N. Switz, C. Reber, J.L. Davis, C. Miller, J.B. Matovu, W. Worodria, L. Huang, D.A. Fletcher, A. Cattamanchi

Automated tuberculosis diagnosis using fluorescence images from a mobile microscope
Med Image Comput Comput Assist Interv. 15, 345-52 (2012)
J. Chang, P. Arbeláez, N. Switz, C. Reber, A. Tapley, J.L. Davis, A. Cattamanchi, D.A. Fletcher, J. Malik

Single-Cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions
Angew. Chem. Int. Ed. Engl. 50, 390–395 (2011)
R. Novak, Y. Zeng, J. Shuga, G. Venugopalan, D.A. Fletcher, M.T. Smith, R.A. Mathies

Mechanics and contraction dynamics of single platelets and implications for clot stiffening
Nature Materials (2011); DOI: 10.1038/nmat2903
Lam WA, Chaudhuri O, Crow A, Webster KD, Li T, Kita A, Huang J, Fletcher DA

Mobile phone based clinical microscopy for global health applications
PLoS ONE. 4, e6320 (2009)
D.N. Breslauer, R.N. Maamari, N.A. Switz, W.A. Lam, D.A. Fletcher

Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation
Lab Chip. 9, 2003–2009 (2009)
J.C. Stachowiak, D.L. Richmond, T.H. Li, F. Brochard-Wyart and D.A. Fletcher

Dynamic control of needle-free jet injection
J Control Release. 135, 104–112 (2009)
J.C. Stachowiak, T.H. Li, A. Arora, S. Mitragotri, D.A. Fletcher

Mixing solutions in inkjet formed vesicles
Meth. Enzymol. 465, 75–94 (2009)
T.H. Li, J.C. Stachowiak, D.A. Fletcher

Unilamellar vesicle formation and encapsulation by microfluidic jetting
PNAS (2008); DOI: 10.1073/pnas.0710875105
J.C. Stachowiak, D.L. Richmond, T.H. Li, A.P. Liu, S.H. Parekh, D.A. Fletcher

Piezoelectric control of needle-free transdermal drug delivery
J Control Release. 124, 88–97 (2007)
J.C. Stachowiak, M.G. von Muhlen, T.H. Li, L. Jalilian, S.H. Parekh, D.A. Fletcher

Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point spread function
J Opt Soc Am A Opt Image Sci Vis. 24, 2622–2627 (2007)
J.W. Shaevitz, D.A. Fletcher

Differential force microscope for long time-scale biophysical measurements
Rev Sci Instrum. 78, 043711 (2007)
J.L. Choy, S.H. Parekh, O. Chaudhuri, A.P. Liu, C. Bustamante, M.J. Footer, J.A. Theriot, D.A. Fletcher

Needle-free delivery of macromolecules across the skin by nanoliter-volume pulsed microjets
PNAS (2007); DOI: 10.1073/pnas.0700182104
Arora A, Hakim I, Baxter J, Rathnasingham R, Srinivasan R, Fletcher DA, Mitragotri S.

Thermal microscopy with a microfabricated solid immersion lens
Microscale Thermophysical Engineering. 7, 4 267-273 (2003)
D.A. Fletcher, G.S. Kino, and K.E. Goodson

Intravascular drug delivery with the pulsed liquid microjet
Arch. Ophthalmol. 120, 1206–1208 (2002)
D.A. Fletcher, D.V. Palanker, P. Huie, J. Miller, M.F. Marmor, and M.S. Blumenkranz

Micromachined silicon nitride solid immersion lens
JMEMS.  11, 470-478 (2002)
K.B. Crozier, D.A. Fletcher, G.S. Kino, and C.F. Quate

Microfabricated silicon solid immersion lens
JMEMS. 10, 450-459 (2001)
D.A. Fletcher, K.B. Crozier, K.W. Guarini, S.C. Minne, G.S. Kino, C.F. Quate, and K. E. Goodson

Refraction contrast imaging with a scanning microlens
Applied Physics Letters. 78, 3589-3591 (2001)
D.A. Fletcher, K.B. Crozier, C.F. Quate, G.S. Kino, K.E. Goodson, D. Simanovskii, and D.V. Palanker

Focusing in microlenses close to a wavelength in diameter
Opt Lett. 26, 399–401 (2001)
D.A. Fletcher, K.E. Goodson, and G.S. Kino

Pulsed liquid microjet for microsurgery
Applied Physics Letters. 78, 1933-1935 (2001)
D. A. Fletcher and D.V. Palanker

Near-field infrared imaging with a microfabricated solid immersion lens
Applied Physics Letters. 77, 2109-2111 (2000)
D.A. Fletcher, K.B. Crozier, G.S. Kino, C.F. Quate, K.E. Goodson, D. Simanovskii, and D.V. Palanker

Dual integrated actuators for extended range high speed atomic force microscopy
Applied Physics Letters. 75, 1637-1639 (1999)
T. Sulchek, S.C. Mines, J.D. Adams, D.A. Fletcher, A. Atalar, C.F. Quate, and D.M. Adderton