Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
Cell (2021); DOI: 10.1016/j.cell.2020.12.001
Fozouni P, Son S, Diaz de Leon Derby M, Knott GJ, Gray CN, D’Ambrosio MV, Zhao C, Switz NA, Kumar GR, Stephens SI, Boehm D, Tsou CL, Shu J, Bhuiya A, Armstrong M, Harris A, Osterloh JM, Meyer-Franke A, Langelier C, Pollard KS, Crawford ED, Puschnik AS, Phelps M, Kistler A, DeRisi JL, Doudna JA, Fletcher DA, Ott M
Publications
Imaging & Biomedical Instrumentation
Low-Fidelity Assembly of Influenza A Virus Promotes Escape from Host Cells.
Cell. 2018 Nov 29. pii: S0092-8674(18)31455-7.
Vahey MD, Fletcher DA
A Smartphone-Based Tool for Rapid, Portable, and Automated Wide-Field Retinal Imaging.
Transl Vis Sci Technol. 2018 Oct 1;7(5):21.
Kim TN, Myers F, Reber C, Loury PJ, Loumou P, Webster D, Echanique C, Li P, Davila JR, Maamari RN, Switz NA, Keenan J, Woodward MA, Paulus YM, Margolis T, Fletcher DA.
In Southern Nigeria Loa loa Blood Microfilaria Density is Very Low Even in Areas with High Prevalence of Loiasis: Results of a Survey Using the New LoaScope Technology.
Am J Trop Med Hyg. (2018) doi: 10.4269/ajtmh.18-0163.
Emukah E, Rakers LJ, Kahansim B, Miri ES, Nwoke BEB, Griswold E, Saka Y, Anagbogu I, Davies E, Ityonzughul C, D’Ambrosio M, Bakalar M, Fletcher DA, Nutman T, Kamgno J, Richards FO
A Test-and-Not-Treat Strategy for Onchocerciasis in Loa loa–Endemic Areas
N Engl J Med (2017); 377:2044-2052
Kamgno J, Pion SD, Chesnais CB, Bakalar MH, D’Ambrosio MV, Mackenzie CD, Nana-Djeunga HC, Gounoue-Kamkumo R, Njitchouang GR, Nwane P, Tchatchueng-Mbouga JB, Wanji S, Stolk WA, Fletcher DA, Klion AD, Nutman TB, and Boussinesq M
Accuracy of Mobile Phone and Handheld Light Microscopy for the Diagnosis of Schistosomiasis and Intestinal Protozoa Infections in Côte d’Ivoire
PLoS Negl Trop Dis. 10(6), eCollection (2016)
Coulibaly JT, Ouattara M, D’Ambrosio MV, Fletcher DA, Keiser J, Utzinger J, N’Goran EK, Andrews JR, Bogoch II
Evaluation of mobile digital light-emitting diode fluorescence microscopy in Hanoi, Viet Nam
Int J Tuberc Lung Dis. 19(9), 1068-72 (2015)
Chaisson LH, Reber C, Phan H, Switz N, Nilsson LM, Myers F, Nhung NV, Luu L, Pham T, Vu C, Nguyen H, Nguyen A, Dinh T, Nahid P, Fletcher DA, Cattamanchi A.
Assessment of a Smartphone Otoscope Device for the Diagnosis and Management of Otitis Media
Clin Pediatr (Phila). pii: 0009922815593909. [Epub ahead of print], (2015)
Rappaport KM, McCracken CC, Beniflah J, Little WK, Fletcher DA, Lam WA, Shane AL.
Multi-Contrast Imaging and Digital Refocusing on a Mobile Microscope with a Domed LED Array
PLoS One. 10(5), eCollection (2015)
Phillips ZF, D’Ambrosio MV, Tian L, Rulison JJ, Patel HS, Sadras N, Gande AV, Switz NA, Fletcher DA, Waller L.
Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope
Sci Transl Med. 7(286), 286re4. (2015)
M.V. D’Ambrosio, M. Bakalar, S. Bennuru, C. Reber, A. Skandarajah, L. Nilsson, N. Switz, J. Kamgno, S. Pion, M. Boussinesq, T.B. Nutman, D.A. Fletcher
Diagnosis of Schistosoma haematobium Infection with a Mobile Phone-Mounted Foldscope and a Reversed-Lens CellScope in Ghana
Am J Trop Med Hyg. 14-0741 Epub ahead of print (2015)
R.K. Ephraim, E. Duah, J.S. Cybulski, M. Prakash, M.V. D’Ambrosio, D.A. Fletcher, J. Keiser, J.R. Andrews, I.I. Bogoch
A lateral electrophoretic flow diagnostic assay
Lab Chip. 15, 1488-1496 (2015)
R. Lin, A. Skandarajah, R.E. Gerver, H.D. Neira, D.A. Fletcher, A.E. Herr
Novel telemedicine device for diagnosis of corneal abrasions and ulcers in resource-poor settings
JAMA Ophthalmol. 132, 894–895 (2014)
R.N. Maamari, S. Ausayakhun, T.P. Margolis, D.A. Fletcher, J.D. Keenan
Low-cost mobile phone microscopy with a reversed mobile phone camera lens
PLoS ONE. 9, e95330 (2014)
N.A. Switz, M.V. D’Ambrosio, D.A. Fletcher
Quantitative imaging with a mobile phone microscope
PLoS ONE. 9, e96906 (2014)
A. Skandarajah, C.D. Reber, N.A. Switz, D.A. Fletcher
Lipid bilayer vesicle generation using microfluidic jetting
J Vis Exp. 84, e51510 (2014). doi:10.3791/51510
C.W. Coyne, K. Patel, J. Heureaux, J. Stachowiak, D.A. Fletcher, A.P. Liu
A mobile phone-based retinal camera for portable wide field imaging
Br J Ophthalmol. 98, 438–441 (2014)
R.N. Maamari, J.D. Keenan, D.A. Fletcher, T.P. Margolis
Mobile Digital Fluorescence Microscopy for Diagnosis of Tuberculosis
J. Clin. Microbiol. 51, 1774–1778 (2013)
A. Tapley, N. Switz, C. Reber, J.L. Davis, C. Miller, J.B. Matovu, W. Worodria, L. Huang, D.A. Fletcher, A. Cattamanchi
Automated tuberculosis diagnosis using fluorescence images from a mobile microscope
Med Image Comput Comput Assist Interv. 15, 345-52 (2012)
J. Chang, P. Arbeláez, N. Switz, C. Reber, A. Tapley, J.L. Davis, A. Cattamanchi, D.A. Fletcher, J. Malik
In vitro modeling of the microvascular occlusion and thrombosis that occur in hematologic diseases using microfluidic technology
J. Clin. Invest. 122, 408–418 (2012)
M. Tsai, A. Kita, J. Leach, R. Rounsevell, J.N. Huang, J. Moake, R.E. Ware, D.A. Fletcher, W.A. Lam
Forming giant vesicles with controlled membrane composition, asymmetry, and contents
Proc. Natl. Acad. Sci. U.S.A. 108, 9431–9436 (2011)
D.L. Richmond, E.M. Schmid, S. Martens, J.C. Stachowiak, N. Liska, D.A. Fletcher
An AFM-based stiffness clamp for dynamic control of rigidity
PLoS ONE. 6, e17807 (2011)
K. Webster, A. Crow, D.A. Fletcher
Single-Cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions
Angew. Chem. Int. Ed. Engl. 50, 390–395 (2011)
R. Novak, Y. Zeng, J. Shuga, G. Venugopalan, D.A. Fletcher, M.T. Smith, R.A. Mathies
Mechanics and contraction dynamics of single platelets and implications for clot stiffening
Nat Mater. 10, 61–66 (2011)
W.A. Lam, O.Chaudhuri, A. Crow, K.D. Webster, T. Li, A. Kita, J. Huang, D.A. Fletcher
Mobile phone based clinical microscopy for global health applications
PLoS ONE. 4, e6320 (2009)
D.N. Breslauer, R.N. Maamari, N.A. Switz, W.A. Lam, D.A. Fletcher
Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation
Lab Chip. 9, 2003–2009 (2009)
J.C. Stachowiak, D.L. Richmond, T.H. Li, F. Brochard-Wyart and D.A. Fletcher
Dynamic control of needle-free jet injection
J Control Release. 135, 104–112 (2009)
J.C. Stachowiak, T.H. Li, A. Arora, S. Mitragotri, D.A. Fletcher
Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells
Nat. Methods. 6, 383–387 (2009)
O. Chaudhuri, S.H. Parekh, W.A. Lam, D.A. Fletcher
Mixing solutions in inkjet formed vesicles
Meth. Enzymol. 465, 75–94 (2009)
T.H. Li, J.C. Stachowiak, D.A. Fletcher
DNA-coated AFM cantilevers for the investigation of cell adhesion and the patterning of live cells
Angew. Chem. Int. Ed. Engl. 47, 8473–8477 (2008)
S.C. Hsiao, A.K. Crow, W.A. Lam, C.R. Bertozzi, D.A. Fletcher, M.B. Francis
Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry
Lab Chip. 8, 1062–1070 (2008)
M.J. Rosenbluth, W.A. Lam, D.A. Fletcher
Unilamellar vesicle formation and encapsulation by microfluidic jetting
Proc. Natl. Acad. Sci. U.S.A. 105, 4697–4702 (2008)
J.C. Stachowiak, D.L. Richmond, T.H. Li, A.P. Liu, S.H. Parekh, D.A. Fletcher
Piezoelectric control of needle-free transdermal drug delivery
J Control Release. 124, 88–97 (2007)
J.C. Stachowiak, M.G. von Muhlen, T.H. Li, L. Jalilian, S.H. Parekh, D.A. Fletcher
Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point spread function
J Opt Soc Am A Opt Image Sci Vis. 24, 2622–2627 (2007)
J.W. Shaevitz, D.A. Fletcher
Differential force microscope for long time-scale biophysical measurements
Rev Sci Instrum. 78, 043711 (2007)
J.L. Choy, S.H. Parekh, O. Chaudhuri, A.P. Liu, C. Bustamante, M.J. Footer, J.A. Theriot, D.A. Fletcher
Needle-free delivery of macromolecules across the skin by nanoliter-volume pulsed microjets
Proc. Natl. Acad. Sci. U.S.A. 104, 4255–4260 (2007)
Arora A, Hakim I, Baxter J, Rathnasingham R, Srinivasan R, Fletcher DA, Mitragotri S.
Photopatterning of actin filament structures
Nano Lett. 5, 625–628 (2005)
A.P. Liu, D.A.Fletcher
Thermal microscopy with a microfabricated solid immersion lens
Microscale Thermophysical Engineering. 7, 4 267-273 (2003)
D.A. Fletcher, G.S. Kino, and K.E. Goodson
Intravascular drug delivery with the pulsed liquid microjet
Arch. Ophthalmol. 120, 1206–1208 (2002)
D.A. Fletcher, D.V. Palanker, P. Huie, J. Miller, M.F. Marmor, and M.S. Blumenkranz
Micromachined silicon nitride solid immersion lens
JMEMS. 11, 470-478 (2002)
K.B. Crozier, D.A. Fletcher, G.S. Kino, and C.F. Quate
Microfabricated silicon solid immersion lens
JMEMS. 10, 450-459 (2001)
D.A. Fletcher, K.B. Crozier, K.W. Guarini, S.C. Minne, G.S. Kino, C.F. Quate, and K. E. Goodson
Refraction contrast imaging with a scanning microlens
Applied Physics Letters. 78, 3589-3591 (2001)
D.A. Fletcher, K.B. Crozier, C.F. Quate, G.S. Kino, K.E. Goodson, D. Simanovskii, and D.V. Palanker
Focusing in microlenses close to a wavelength in diameter
Opt Lett. 26, 399–401 (2001)
D.A. Fletcher, K.E. Goodson, and G.S. Kino
Pulsed liquid microjet for microsurgery
Applied Physics Letters. 78, 1933-1935 (2001)
D. A. Fletcher and D.V. Palanker
Near-field infrared imaging with a microfabricated solid immersion lens
Applied Physics Letters. 77, 2109-2111 (2000)
D.A. Fletcher, K.B. Crozier, G.S. Kino, C.F. Quate, K.E. Goodson, D. Simanovskii, and D.V. Palanker
Dual integrated actuators for extended range high speed atomic force microscopy
Applied Physics Letters. 75, 1637-1639 (1999)
T. Sulchek, S.C. Mines, J.D. Adams, D.A. Fletcher, A. Atalar, C.F. Quate, and D.M. Adderton