Publications
Bead-based RNA Detection with Cas13a: Multi-scale Design and Validation
Biorxiv (2026); DOI: 10.64898/2026.07.27.739218
Bhuiya AM, Agarwal S, Ng CF, Ott M, Son S, Fletcher DA
Overcoming steric inhibition of antibody-dependent phagocytosis with tall adhesions
Biorxiv (2026); DOI: 10.64898/2026.01.09.698749
Chorlay A*, Joffe AM*, Huzar J, Hasnain J, Geissler PL, Fletcher DA
LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs
Nucleic Acids Research (2026); DOI: 10.1093/nar/gkag848
Ng CF*, Krishnamurthy D*, Dextre A, Chorlay A, Ott M, Fletcher DA
Programmable kinetic barcoding for multiplexed RNA detection with Cas13a
Nature Biomedical Engineering (2026); DOI: 10.1038/s41551-026-01642-6
Son S, Lyden A, Ng CF, Dextre A, Shu J, Stephens SI, Fozouni P, Knott GJ, Smock DCJ, Liu TY, Boehm D, Simoneau C, Kumar GR, Doudna JA, Ott M, Fletcher DA
NTDscope: A multi-contrast portable microscope for disease diagnosis
PLOS Global Public Health (2026); DOI: 10.1371/journal.pgph.0005937
Díaz de León Derby M, Moussa ZL, Ng CF, Bhuiya AM, Cabrera JP, Banik D, Delahunt CB, Keller MD, Le Ny ALM, Garcia-Villena J, Dacal E, Bermejo-Peláez D, Cuadrado D, Luengo-Oroz M, Djeunga HCN, Kamgno J, Yemeli LD, Pahl V, Davi SD, Manego RZ, Ramharter M, Bogoch II, Coulibaly JT, Khatun A, Kabir M, Noor Z, Haque R, Switz NA, Friedman DH, D’Ambrosio MV, Fletcher DA
Target cell cortical tension regulates macrophage trogocytosis
Nature Cell Biology (2025); DOI: 10.1038/s41556-025-01807-6
Cornell CE, Chorlay A, Krishnamurthy D, Martin NR, Baldauf L, Fletcher DA
SurFlex microscopy: Measuring flexibility of surface-tethered biomolecules
PNAS (2025); DOI: 10.1073/pnas.2508828122
Chorlay A, Agarwal S, Blackmon L, Dextre A, Fletcher DA
Cell surface crowding is a tunable energetic barrier to cell-cell fusion
Nature Communications (2025); DOI: 10.1038/s41467-025-62330-8
Lee DSW*, Oster LF*, Son S, Fletcher DA
Multi-contrast machine learning improves schistosomiasis diagnostic performance
PLOS Neglected Tropical Diseases (2025); DOI: 10.1371/journal.pntd.0012879
Díaz de León Derby M, Delahunt CB, Spencer E, Coulibaly JT, Silue KD, Bogoch II, Le Ny ALM, Fletcher DA
Challenges of microscopy technology dissemination to resource-constrained communities
Nature Methods (2025); DOI: 10.1038/s41592-025-02690-7
Aaron JS, Jacobs AC, Malacrida L, Keppler A, French P, Fletcher DA, Wood C, Brown CM, Wright GD, Ogawa S, Maina M, Chew T-L
Kinetics and Optimality of Influenza A Virus Locomotion
Physical Review Letters (2024); DOI: 10.1103/PhysRevLett.133.248402
Agarwal S, Veytsman B, Fletcher DA, Huber G
Engineered molecular sensors for quantifying cell surface crowding
PNAS (2023); DOI: 10.1073/pnas.2219778120
Takatori SC*, Son S*, Lee DSW, Fletcher DA
Programming multicellular assembly with synthetic cell adhesion molecules
Nature (2022); DOI: 10.1038/s41586-022-05622-z
Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
The molecular mechanism of load adaptation by branched actin networks
eLife (2022); DOI: 10.7554/eLife.73145
Li TD*, Bieling P*, Weichsel J, Mullins RD, Fletcher DA
Point-of-Care Sample Preparation and Automated Quantitative Detection of Schistosoma haematobium Using Mobile Phone Microscopy
American Journal of Tropical Medicine and Hygiene (2022); DOI: 10.4269/ajtmh.21-1071
Armstrong M, Harris AR, D’Ambrosio MV, Coulibaly JT, Essien-Baidoo S, Ephraim RKD, Andrews JR, Bogoch II, Fletcher DA
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
Cell (2021); DOI: 10.1016/j.cell.2020.12.001
Fozouni P*, Son S*, Díaz de León Derby M*, Knott GJ, Gray CN, D’Ambrosio MV, Zhao C, Switz NA, Kumar GR, Stephens SI, Boehm D, Tsou CL, Shu J, Bhuiya A, Armstrong M, Harris A, Osterloh JM, Meyer-Franke A, Langelier C, Pollard KS, Crawford ED, Puschnik AS, Phelps M, Kistler A, DeRisi JL, Doudna JA, Fletcher DA, Ott M
Evolutionarily related small viral fusogens hijack distinct but modular actin nucleation pathways to drive cell-cell fusion
PNAS (2021); DOI: 10.1073/pnas.2007526118
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Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
Cell Reports (2021); DOI: 10.1016/j.celrep.2021.109587
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Cell–cell interfaces as specialized compartments directing cell function
Nature Reviews Molecular Cell Biology (2020); DOI: 10.1038/s41580-020-00298-7
, Son S, Felce JH, Dustin ML,
Forming and loading giant unilamellar vesicles with acoustic jetting
Biomicrofluidics (2020); DOI: 10.1063/5.0021742
Armstrong M, Vahey MD, Hunt TP, Fletcher DA
A Weak Link with Actin Organizes Tight Junctions to Control Epithelial Permeability
Developmental Cell (2020); DOI: 10.1016/j.devcel.2020.07.022
, Hamkins-Indik T*, Harris AR, Kim J, Xu K,
Macrophage phagocytosis assay with reconstituted target particles
Nature Protocols (2020); DOI: 10.1038/s41596-020-0330-8
Joffe AM, Bakalar MH, Fletcher DA
Molecular height measurement by cell surface optical profilometry (CSOP)
PNAS (2020); DOI: 10.1073/pnas.1922626117
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Biased localization of actin binding proteins by actin filament conformation
Nature Communications (2020); DOI: 10.1038/s41467-020-19768-9
Harris AR, Jreij P, Belardi B, Joffe AM, Bausch AR, Fletcher DA
Microscale fluid behavior during cryo-EM sample blotting
Biophysical Journal (2020); DOI: 10.1016/j.bpj.2019.12.017
Armstrong M, Han B-G, Gomez S, Turner J, Fletcher DA, Glaeser RM
A viral fusogen hijacks the actin cytoskeleton to drive cell-cell fusion
eLife (2020); DOI: 10.7554/eLife.51358
Chan KMC, Son S, Schmid EM, Fletcher DA
Steric Regulation of Tandem Calponin Homology Domain Actin-Binding Affinity
Molecular Biology of the Cell (2019); DOI: 10.1091/mbc.E19-06-0317
Harris AR, Belardi B, Jreij P, Wei K, Shams H, Bausch A, Fletcher DA
Deep learning for single-shot autofocus microscopy
Optica (2019); DOI: 10.1364/OPTICA.6.000794
Pinkard H, Phillips Z, Babakhani A, Fletcher DA, Waller L
Smartphone-Based, Rapid, Wide-Field Fundus Photography for Diagnosis of Pediatric Retinal Diseases
Translational Vision Science & Technology (2019); DOI: 10.1167/tvst.8.3.29
Patel TP, Kim TN, Yu G, Dedania VS, Lieu P, Qian CX, Besirli CG, Demirci H, Margolis T, Fletcher DA, Paulus YM
Influenza A virus surface proteins are organized to help penetrate host mucus
eLife (2019); DOI: 10.7554/eLife.43764
Vahey MD, Fletcher DA
Stiffness Sensing by Cells
Physiological Reviews (2019); DOI: 10.1152/physrev.00013.2019
In pursuit of the mechanics that shape cell surfaces
Nature Physics (2018); DOI: 10.1038/s41567-018-0187-8
Diz-Muñoz A, Weiner DO, Fletcher DA
Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane
Journal of Biological Chemistry (2018); DOI: 10.1074/jbc.RA118.005669
Lakshman B, Messing S, Schmid EM, Clogston JD, Gillette WK, Esposito D, Kessing B, Fletcher DA, Nissley DV, McCormick F, Stephen AG, Jean-Francois FL
Low-Fidelity Assembly of Influenza A Virus Promotes Escape from Host Cells
Cell (2018); DOI: 10.1016/j.cell.2018.10.056
Vahey MD, Fletcher DA
Which biological systems should be engineered?
Nature (2018)
Fletcher DA
From solution to surface to filament: actin flux into branched networks
Biophysical Reviews (2018); DOI: 10.1007/s12551-018-0469-5
Mullins RD, Bieling P, Fletcher DA
A Smartphone-Based Tool for Rapid, Portable, and Automated Wide-Field Retinal Imaging
Translational Vision Science & Technology (2018); DOI: 10.1167/tvst.7.5.21
Kim TN, Myers F, Reber C, Loury PJ, Loumou P, Webster D, Echanique C, Li P, Davila JR, Maamari RN, Switz NA, Keenan J, Woodward MA, Paulus YM, Margolis T, Fletcher DA
Mechanotransduction by the Actin Cytoskeleton: Converting Mechanical Stimuli into Biochemical Signals
Annual Review of Biophysics (2018); DOI: 10.1146/annurev-biophys-070816-033547
Harris AR, Jreij P, Fletcher DA
One and one is not two: taking a fresh look at membrane interfaces
Nature Reviews Molecular Cell Biology (2018); DOI: 10.1038/s41580-018-0057-3
Belardi B, Fletcher DA
Claudin-4 reconstituted in unilamellar vesicles is sufficient to form tight interfaces that partition membrane proteins
Journal of Cell Science (2018); DOI: 10.1242/jcs.221556
Belardi B, Son S, Vahey MD, Wang J, Hou J, Fletcher DA
Size-Dependent Segregation Controls Macrophage Phagocytosis of Antibody-Opsonized Targets
Cell (2018); DOI: 10.1016/j.cell.2018.05.059
Bakalar MH, Joffe AM, Schmid EM, Son S, Podolski M, Fletcher DA
Spectrin is a mechanoresponsive protein shaping fusogenic synapse architecture during myoblast fusion
Nature Cell Biology (2018); DOI: 10.1038/s41556-018-0106-3
Duan R, Kim JH, Shilagardi K, Schiffhauer ES, Lee DM, Son S, Li S, Thomas C, Luo T, Fletcher DA, Robinson DN, Chen EH
Transient external force induces phenotypic reversion of malignant epithelial structures via nitric oxide signaling
eLife (2018); DOI: 10.7554/eLife.26161
Ricca BL*, Venugopalan G*, Furuta S, Tanner K, Orellana WA, Reber CD, Brownfield DG, Bissell MJ, Fletcher DA
In Southern Nigeria Loa loa Blood Microfilaria Density is Very Low Even in Areas with High Prevalence of Loiasis: Results of a Survey Using the New LoaScope Technology
American Journal of Tropical Medicine and Hygiene (2018); DOI: 10.4269/ajtmh.18-0163
Emukah E, Rakers LJ, Kahansim B, Miri ES, Nwoke BEB, Griswold E, Saka Y, Anagbogu I, Davies E, Ityonzughul C, D’Ambrosio M, Bakalar M, Fletcher DA, Nutman T, Kamgno J, Richards FO
A Test-and-Not-Treat Strategy for Onchocerciasis in Loa loa–Endemic Areas
New England Journal of Medicine (2017); DOI: 10.1056/NEJMoa1705026
Kamgno J, Pion SD, Chesnais CB, Bakalar MH, D’Ambrosio MV, Mackenzie CD, Nana-Djeunga HC, Gounoue-Kamkumo R, Njitchouang GR, Nwane P, Tchatchueng-Mbouga JB, Wanji S, Stolk WA, Fletcher DA, Klion AD, Nutman TB, and Boussinesq M
Force-induced transcellular tunnel formation in endothelial cells
Molecular Biology of the Cell (2017); DOI: 10.1091/mbc.E17-01-0080
Ng WP, Webster KD, Stefani C, Schmid EM, Lemichez E, Bassereau, Fletcher DA
Organ sculpting by patterned extracellular matrix stiffness
eLife (2017); DOI: 10.7554/eLife.24958.001
Crest J, Diz-Muñoz A, Chen DY, Fletcher DA, Bilder D
Bottom-Up Biology: Harnessing Engineering to Understand Nature
Developmental Cell (2016); DOI: 10.1016/j.devcel.2016.09.009
Fletcher DA
Accuracy of Mobile Phone and Handheld Light Microscopy for the Diagnosis of Schistosomiasis and Intestinal Protozoa Infections in Côte d’Ivoire
PLoS Neglected Tropical Diseases (2016); DOI: 10.1371/journal.pntd.0004768
Coulibaly JT, Ouattara M, D’Ambrosio MV, Fletcher DA, Keiser J, Utzinger J, N’Goran EK, Andrews JR, Bogoch II
Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration
PLoS Biology (2016); DOI: 10.1371/journal.pbio.1002474
Diz-Muñoz A, Thurley K, Chintamen S, Altschuler SJ, Wu LF, Fletcher DA, Weiner OD
Size-dependent protein segregation at membrane interfaces
Nature Physics (2016); DOI: 10.1038/nphys3678
Schmid EM*, Bakalar MH*, Choudhuri K*, Weichsel J, Ann HS, Geissler PL, Dustin ML, Fletcher DA
Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks
Cell (2016); DOI: 10.1016/j.cell.2015.11.057
Bieling P*, Li TD*, Weichsel J, McGorty R, Jreij P, Huang B, Fletcher DA, Mullins RD
Evaluation of mobile digital light-emitting diode fluorescence microscopy in Hanoi, Viet Nam
International Journal of Tuberculosis and Lung Disease (2015); DOI: 10.5588/ijtld.15.0018
Chaisson LH, Reber C, Phan H, Switz N, Nilsson LM, Myers F, Nhung NV, Luu L, Pham T, Vu C, Nguyen H, Nguyen A, Dinh T, Nahid P, Fletcher DA, Cattamanchi A
Assessment of a Smartphone Otoscope Device for the Diagnosis and Management of Otitis Media
Clinical Pediatrics (2015); DOI: 10.1177/0009922815593909
Rappaport KM, McCracken CC, Beniflah J, Little WK, Fletcher DA, Lam WA, Shane AL.
Reconstitution of proteins on electroformed unilamellar vesicles
Methods in Cell Biology (2015); DOI: 10.1016/bs.mcb.2015.02.004
Schmid EM, Richmond DL, Fletcher DA
Multi-Contrast Imaging and Digital Refocusing on a Mobile Microscope with a Domed LED Array
PLoS One. 10(5), eCollection (2015)
Phillips ZF, D’Ambrosio MV, Tian L, Rulison JJ, Patel HS, Sadras N, Gande AV, Switz NA, Fletcher DA, Waller L.
Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope
Science Translational Medicine (2015); DOI: 10.1126/scitranslmed.aaa3480
D’Ambrosio MV, Bakalar M, Bennuru S, Reber C, Skandarajah A, Nilsson L, Switz N, Kamgno J, Pion S, Boussinesq M, Nutman TB, Fletcher DA
Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase
J Cell Biol. (2015); DOI: 10.1083/jcb.201409001
Lou SS, Diz-Muñoz A, Weiner OD, Fletcher DA, Theriot JA
Diagnosis of Schistosoma haematobium Infection with a Mobile Phone-Mounted Foldscope and a Reversed-Lens CellScope in Ghana
American Journal of Tropical Medicine and Hygiene (2015); DOI: 10.4269/ajtmh.14-0741
Ephraim RK, Duah E, Cybulski JS, Prakash M, D’Ambrosio MV, Fletcher DA, Keiser J, Andrews JR, Bogoch II
Mechanical tension drives cell membrane fusion
Developmental Cell (2015); DOI: 10.1016/j.devcel.2015.01.005
Kim JH, Ren Y, Ng WP, Li S, Son S, Kee YS, Zhang S, Zhang G, Fletcher DA, Robinson DN, Chen EH
A lateral electrophoretic flow diagnostic assay
Lab Chip (2015); DOI: 10.1039/c4lc01370k
Lin R, Skandarajah A, Gerver RE, Neira HD, Fletcher DA, Herr AE
Multicellular architecture of malignant breast epithelia influences mechanics
PLOS ONE (2014); DOI: 10.1371/journal.pone.0101955
Venugopalan G, Camarillo DB, Webster KD, Reber CD, Sethian JA, Weaver VM, Fletcher DA, El-Samad H, Rycroft CH
Novel telemedicine device for diagnosis of corneal abrasions and ulcers in resource-poor settings
JAMA Ophthalmol. 132, 894–895 (2014)
R.N. Maamari, S. Ausayakhun, T.P. Margolis, D.A. Fletcher, J.D. Keenan
Tensional homeostasis in single fibroblasts
Biophysical Journal (2014); DOI: 10.1016/j.bpj.2014.04.051
Webster KD, Ng WP, Fletcher DA
Low-cost mobile phone microscopy with a reversed mobile phone camera lens
PLOS ONE (2014); DOI: 10.1371/journal.pone.0095330
Switz NA, D’Ambrosio MV, Fletcher DA
Dynamic mechanical responses of Arabidopsis thylakoid membranes during PSII-specific illumination
Biophysical Journal (2014); DOI: 10.1016/j.bpj.2014.03.016
Clausen CH, Brooks MD, Li TD, Grob P, Kemalyan G, Nogales E, Niyogi KK, Fletcher DA
Quantitative imaging with a mobile phone microscope
PLOS ONE (2014); DOI: 10.1371/journal.pone.0096906
Skandarajah A, Reber CD, Switz NA, Fletcher DA
Lipid bilayer vesicle generation using microfluidic jetting
J Vis Exp. 84, e51510 (2014). doi:10.3791/51510
C.W. Coyne, K. Patel, J. Heureaux, J. Stachowiak, D.A. Fletcher, A.P. Liu
Micropattern-guided assembly of overlapping pairs of dynamic microtubules
Meth. Enzymol. 540, 339–360 (2014)
F.J. Fourniol, T.D. Li, P. Bieling, R.D. Mullins, D.A. Fletcher, T. Surrey
A mobile phone-based retinal camera for portable wide field imaging
Br J Ophthalmol. 98, 438–441 (2014)
R.N. Maamari, J.D. Keenan, D.A. Fletcher, T.P. Margolis
The biology of boundary conditions: cellular reconstitution in one, two, and three dimensions
Curr. Opin. Cell Biol. (2014); DOI: 10.1016/j.ceb.2013.10.001
Vahey MD, Fletcher DA
Cytoplasmic volume modulates spindle size during embryogenesis
Science (2013); DOI: 10.1126/science.1243147
Good MC, Vahey MD, Skandarajah A, Fletcher DA, Heald R
Placental syncytium forms a biophysical barrier against pathogen invasion
PLOS Pathogens (2013); DOI: 10.1371/journal.ppat.1003821
Zeldovich VB, Clausen CH, Bradford E, Fletcher DA, Maltepe E, Robbins JR, Bakardjiev AI
To pull or be pulled: parsing the multiple modes of mechanotransduction
Curr. Opin. Cell Biol. 25, 558–564 (2013)
B.L. Ricca, G. Venugopalan, D.A. Fletcher
A multi-structural single cell model of force-induced interactions of cytoskeletal components
Biomaterials. 34, 6119–6126 (2013)
S. Barreto, C.H. Clausen, C.M. Perrault, D.A. Fletcher, D. Lacroix
Patterned Collagen Fibers Orient Branching Mammary Epithelium through Distinct Signaling Modules
Curr. Biol. 23, 703–709 (2013)
D.G. Brownfield, G. Venugopalan, A. Lo, H. Mori, K. Tanner, D.A. Fletcher, M.J. Bissell
Mobile Digital Fluorescence Microscopy for Diagnosis of Tuberculosis
J. Clin. Microbiol. 51, 1774–1778 (2013)
A. Tapley, N. Switz, C. Reber, J.L. Davis, C. Miller, J.B. Matovu, W. Worodria, L. Huang, D.A. Fletcher, A. Cattamanchi
Use the force: membrane tension as an organizer of cell shape and motility
Trends in Cell Biology (2013); DOI: 10.1016/j.tcb.2012.09.006
Diz-Muñoz A, Fletcher DA, Weiner OD
Automated tuberculosis diagnosis using fluorescence images from a mobile microscope
Med Image Comput Comput Assist Interv. 15, 345-52 (2012)
J. Chang, P. Arbeláez, N. Switz, C. Reber, A. Tapley, J.L. Davis, A. Cattamanchi, D.A. Fletcher, J. Malik
Membrane bending by protein-protein crowding
Nature Cell Biology (2012); DOI: 10.1038/ncb2561
Stachowiak JC, Schmid EM, Ryan CJ, Ann HS, Sasaki DY, Sherman MB, Geissler PL, Fletcher DA, Hayden CC
Contractile equilibriation of single cells to step changes in extracellular stiffness
Biophysical Journal (2012); DOI: 10.1016/j.bpj.2011.11.4020
Crow A, Webster KD, Hohlfeld E, Ng WP, Geissler P, Fletcher DA
Actin filament curvature biases branching direction
PNAS (2012); DOI: 10.1073/pnas.1114292109
V.I. Risca, E.B. Wang, O. Chaudhuri, J.J. Chia, P.L. Geissler, D.A. Fletcher
In vitro modeling of the microvascular occlusion and thrombosis that occur in hematologic diseases using microfluidic technology
J. Clin. Invest. 122, 408–418 (2012)
M. Tsai, A. Kita, J. Leach, R. Rounsevell, J.N. Huang, J. Moake, R.E. Ware, D.A. Fletcher, W.A. Lam
Microenvironmental geometry guides platelet adhesion and spreading: A quantitative analysis at the single cell level
PLoS ONE. 6, e26437 (2011)
A. Kita, Y. Sakuri, D.R. Myers, R. Rounsevell, J.N. Huang, T.J. Seok, K. Yu, M.C. Wu, D.A. Fletcher, W.A. Lam
Forming giant vesicles with controlled membrane composition, asymmetry, and contents
PNAS (2011); DOI: 10.1073/pnas.1016410108
D.L. Richmond, E.M. Schmid, S. Martens, J.C. Stachowiak, N. Liska, D.A. Fletcher
An AFM-based stiffness clamp for dynamic control of rigidity
PLoS ONE. 6, e17807 (2011)
K. Webster, A. Crow, D.A. Fletcher
Single-Cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions
Angew. Chem. Int. Ed. Engl. 50, 390–395 (2011)
R. Novak, Y. Zeng, J. Shuga, G. Venugopalan, D.A. Fletcher, M.T. Smith, R.A. Mathies
Mechanics and contraction dynamics of single platelets and implications for clot stiffening
Nature Materials (2011); DOI: 10.1038/nmat2903
Lam WA, Chaudhuri O, Crow A, Webster KD, Li T, Kita A, Huang J, Fletcher DA
On force and function
Mol. Biol. Cell. (2010); DOI: 10.1091/mbc.E10-06-0538
Fletcher DA
Large-scale simulations of fluctuating biological membranes
J Chem Phys. 132, 154107 (2010)
A. Pasqua, L. Maibaum, G. Oster, D.A. Fletcher
Molecular Biomechanics: The Molecular Basis of How Forces Regulate Cellular Function
Mol Cell Biomech. 3, 91–105 (2010)
G. Bao, R.D. Kamm, W. Thomas, W. Hwang, D.A. Fletcher, A.J. Grodzinsky, C. Zhu, M.R. Mofrad
Cell mechanics and the cytoskeleton
Nature (2010); DOI: 10.1038/nature08908
Fletcher DA and Mullins RD
Biology under construction: in vitro reconstitution of cellular function
Nature Reviews Molecular Cell Biology (2009); DOI: 10.1038/nrm2746
Liu AP, Fletcher DA
Mobile phone based clinical microscopy for global health applications
PLoS ONE. 4, e6320 (2009)
D.N. Breslauer, R.N. Maamari, N.A. Switz, W.A. Lam, D.A. Fletcher
Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation
Lab Chip. 9, 2003–2009 (2009)
J.C. Stachowiak, D.L. Richmond, T.H. Li, F. Brochard-Wyart and D.A. Fletcher
Active biological materials
Annu Rev Phys Chem. 60, 469–486 (2009)
D.A. Fletcher and P.L. Geissler
Dynamic control of needle-free jet injection
J Control Release. 135, 104–112 (2009)
J.C. Stachowiak, T.H. Li, A. Arora, S. Mitragotri, D.A. Fletcher
Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells
Nature Methods (2009); DOI: 10.1038/nmeth.1320
Chaudhuri O, Parekh SH, Lam WA, Fletcher DA
Mixing solutions in inkjet formed vesicles
Meth. Enzymol. 465, 75–94 (2009)
T.H. Li, J.C. Stachowiak, D.A. Fletcher
Slow Stress Propagation in Adherent Cells
Biophysical Journal (2008); DOI: 10.1529/biophysj.108.139139
Rosenbluth MJ, Crow A, Shaevitz JW, Fletcher DA
DNA-coated AFM cantilevers for the investigation of cell adhesion and the patterning of live cells
Angew. Chem. Int. Ed. Engl. (2008); DOI: 10.1002/anie.200802525
Hsiao SC, Crow AK, Lam WA, Bertozzi CR, Fletcher DA, Francis MB
Membrane-induced bundling of actin filaments
Nature Physics (2008); DOI: 10.1038/nphys1071
Liu AP, Richmond DL, Maibaum L, Pronk S, Geissler PL, Fletcher DA
Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry
Lab Chip (2008); DOI: 10.1039/b802931h
Rosenbluth MJ, Lam WA, Fletcher DA
Limits of filipodium stability
Phys. Rev. Lett. 100, 258102 (2008)
S. Pronk, P.L. Geissler, D.A. Fletcher
Curvature and torsion in growing actin networks
Phys Biol. 5, 026006 (2008)
J.W. Shaevitz, D.A. Fletcher
Increased leukaemia cell stiffness is associated with symptoms of leucostasis in paediatric acute lymphoblastic leukaemia
Br. J. Haematol. 142, 497–501 (2008)
W.A. Lam, M.J. Rosenbluth, D.A. Fletcher
Unilamellar vesicle formation and encapsulation by microfluidic jetting
PNAS (2008); DOI: 10.1073/pnas.0710875105
J.C. Stachowiak, D.L. Richmond, T.H. Li, A.P. Liu, S.H. Parekh, D.A. Fletcher
Tonic shock induces detachment of Giardia lamblia
PLoS Negl Trop Dis. 2, e169 (2008)
W.R. Hansen, D.A. Fletcher
Piezoelectric control of needle-free transdermal drug delivery
J Control Release. 124, 88–97 (2007)
J.C. Stachowiak, M.G. von Muhlen, T.H. Li, L. Jalilian, S.H. Parekh, D.A. Fletcher
Load fluctuations drive actin network growth
PNAS (2007); DOI: 10.1073/pnas.0702601104
Shaevitz JW, Fletcher DA
Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point spread function
J Opt Soc Am A Opt Image Sci Vis. 24, 2622–2627 (2007)
J.W. Shaevitz, D.A. Fletcher
Emergence of large-scale cell morphology and movement from local actin filament growth dynamics
PLoS Biol. 5, e233 (2007)
C.I. Lacayo, Z. Pincus, M.M. van Duijn, C.A. Wilson, D.A. Fletcher, F.B. Gertler, A. Mogilner, J.A. Theriot
Differential force microscope for long time-scale biophysical measurements
Rev Sci Instrum. 78, 043711 (2007)
J.L. Choy, S.H. Parekh, O. Chaudhuri, A.P. Liu, C. Bustamante, M.J. Footer, J.A. Theriot, D.A. Fletcher
Chemotherapy exposure increases leukemia cell stiffness
Blood (2007); DOI: 10.1182/blood-2006-08-043570
Lam WA, Rosenbluth MJ, Fletcher DA
Needle-free delivery of macromolecules across the skin by nanoliter-volume pulsed microjets
PNAS (2007); DOI: 10.1073/pnas.0700182104
Arora A, Hakim I, Baxter J, Rathnasingham R, Srinivasan R, Fletcher DA, Mitragotri S.
Reversible stress softening of actin networks
Nature (2007); DOI: 10.1038/nature05459
Chaudhuri O, Parekh SH, Fletcher DA
Actin polymerization serves as a membrane domain switch in model lipid bilayers
Biophysical Journal (2006); DOI: 10.1529/biophysj.106.090852
Liu AP, Fletcher DA
Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic Cultures
Science (2006); DOI: 10.1126/science.1131000
Nelson CM, Vanduijn MM, Inman JL, Fletcher DA, Bissell MJ
Fast, cheap, and somewhat in control
Genome Biol. 7, 114 (2006)
A.P. Arkin and D.A. Fletcher
Giardia lamblia Attachment Force is Insensitive to Surface Treatments
Eukaryotic Cell. 5, 781–783 (2006)
W.R. Hansen, O Tulyathan, S.C. Dawson, W.Z. Cande, and D. A. Fletcher
Force microscopy of nonadherent cells: A comparison of leukemia cell deformability
Biophysical Journal (2006); DOI: 10.1529/biophysj.105.067496
Rosenbluth MJ, Lam WA, Fletcher DA
Loading history determines velocity of actin network growth
Nature Cell Biology (2005); DOI: 10.1038/ncb1336
Parekh SH, Chaudhuri O, Theriot J, Fletcher DA
Spiroplasma Swim by a Processive Change in Body Helicity
Cell (2005); DOI: 10.1016/j.cell.2005.07.004
Shaevitz JW, Lee JY, Fletcher DA
Photopatterning of actin filament structures
Nano Lett. 5, 625–628 (2005)
A.P. Liu, D.A.Fletcher
An introduction to cell motility for the physical scientist
Phys Biol. 1, T1–10 (2004)
D. A. Fletcher and J. A. Theriot
Differentiation and developmental pathways of uropathogenic E. coli in urinary tract pathogenesis
PNAS (2004); DOI: 10.1073/pnas.0308125100
Justice SS, Hung C, Theriot JA, Fletcher DA, Anderson GG , Footer MJ, Hultgren SJ
Thermal microscopy with a microfabricated solid immersion lens
Microscale Thermophysical Engineering. 7, 4 267-273 (2003)
D.A. Fletcher, G.S. Kino, and K.E. Goodson
Compression forces generated by actin comet tails on lipid vesicles
PNAS (2003); DOI: 10.1073/pnas.1031670100
Giardini PA, Fletcher DA, Theriot JA
Intravascular drug delivery with the pulsed liquid microjet
Arch. Ophthalmol. 120, 1206–1208 (2002)
D.A. Fletcher, D.V. Palanker, P. Huie, J. Miller, M.F. Marmor, and M.S. Blumenkranz
Micromachined silicon nitride solid immersion lens
JMEMS. 11, 470-478 (2002)
K.B. Crozier, D.A. Fletcher, G.S. Kino, and C.F. Quate
Microfabricated silicon solid immersion lens
JMEMS. 10, 450-459 (2001)
D.A. Fletcher, K.B. Crozier, K.W. Guarini, S.C. Minne, G.S. Kino, C.F. Quate, and K. E. Goodson
Refraction contrast imaging with a scanning microlens
Applied Physics Letters. 78, 3589-3591 (2001)
D.A. Fletcher, K.B. Crozier, C.F. Quate, G.S. Kino, K.E. Goodson, D. Simanovskii, and D.V. Palanker
Focusing in microlenses close to a wavelength in diameter
Opt Lett. 26, 399–401 (2001)
D.A. Fletcher, K.E. Goodson, and G.S. Kino
Pulsed liquid microjet for microsurgery
Applied Physics Letters. 78, 1933-1935 (2001)
D. A. Fletcher and D.V. Palanker
Near-field infrared imaging with a microfabricated solid immersion lens
Applied Physics Letters. 77, 2109-2111 (2000)
D.A. Fletcher, K.B. Crozier, G.S. Kino, C.F. Quate, K.E. Goodson, D. Simanovskii, and D.V. Palanker
Dual integrated actuators for extended range high speed atomic force microscopy
Applied Physics Letters. 75, 1637-1639 (1999)
T. Sulchek, S.C. Mines, J.D. Adams, D.A. Fletcher, A. Atalar, C.F. Quate, and D.M. Adderton