Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane.
J Biol Chem. 2018 Dec 17. pii: jbc.RA118.005669.
Lakshman B, Messing S, Schmid EM, Clogston JD, Gillette WK, Esposito D, Kessing B, Fletcher DA, Nissley DV, McCormick F, Stephen AG, Jean-Francois FL
Publications
In Vitro Reconstitution
Low-Fidelity Assembly of Influenza A Virus Promotes Escape from Host Cells.
Cell. 2018 Nov 29. pii: S0092-8674(18)31455-7.
Vahey MD, Fletcher DA
One and one is not two: taking a fresh look at membrane interfaces.
Nat Rev Mol Cell Biol. (2018) Sep 6. doi: 10.1038/s41580-018-0057-3. [Epub ahead of print]
Belardi B, Fletcher DA.
Claudin-4 reconstituted in unilamellar vesicles is sufficient to form tight interfaces that partition membrane proteins.
J Cell Sci. (2018) Sep 12. 10.1242/jcs.221556. [Epub ahead of print]
Belardi B, Son S, Vahey MD, Wang J, Hou J, Fletcher DA.
Size-Dependent Segregation Controls Macrophage Phagocytosis of Antibody-Opsonized Targets.
Cell. (2018) Jun 28;174(1):131-142.e13.
Bakalar MH, Joffe AM, Schmid EM, Son S, Podolski M, Fletcher DA
Bottom-Up Biology: Harnessing Engineering to Understand Nature.
Dev Cell. 26;38(6):587-9 (2016)
Fletcher DA
Size-dependent protein segregation at membrane interfaces
Nature Physics. Published online: 7 March 2016 | doi: 10.1038/nphys3678
Eva M. Schmid , Matthew H. Bakalar, Kaushik Choudhuri, Julian Weichsel, Hyoung Sook Ann, Phillip L. Geissler, Michael L. Dustin and Daniel A. Fletcher
Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks
Cell. 164(1-2), 115-27 (2016)
Bieling P, Li TD, Weichsel J, McGorty R, Jreij P, Huang B, Fletcher DA, Mullins RD.
Reconstitution of proteins on electroformed unilamellar vesicles
Methods Cell Biol. 128:319-38. (2015)
E.M. Schmid, D.L. Richmond, D.A. Fletcher
Micropattern-guided assembly of overlapping pairs of dynamic microtubules
Meth. Enzymol. 540, 339–360 (2014)
F.J. Fourniol, T.D. Li, P. Bieling, R.D. Mullins, D.A. Fletcher, T. Surrey
The biology of boundary conditions: cellular reconstitution in one, two, and three dimensions
Curr. Opin. Cell Biol. 26, 60–68 (2014)
M.D. Vahey, D.A. Fletcher
Cytoplasmic volume modulates spindle size during embryogenesis
Science. 342, 856–860 (2013)
M.C. Good, M.D. Vahey, A. Skandarajah, D.A. Fletcher, R. Heald
Membrane bending by protein-protein crowding
Nat. Cell Biol. 14, 944–949 (2012)
J.C. Stachowiak, E.M. Schmid , C.J. Ryan, H.S. Ann, D.Y. Sasaki, M.B. Sherman, P.L. Geissler, D.A. Fletcher, C.C. Hayden
Actin filament curvature biases branching direction
Proc. Natl. Acad. Sci. U.S.A. 109, 2913–2918 (2012)
V.I. Risca, E.B. Wang, O. Chaudhuri, J.J. Chia, P.L. Geissler, D.A. Fletcher
Forming giant vesicles with controlled membrane composition, asymmetry, and contents
Proc. Natl. Acad. Sci. U.S.A. 108, 9431–9436 (2011)
D.L. Richmond, E.M. Schmid, S. Martens, J.C. Stachowiak, N. Liska, D.A. Fletcher
Large-scale simulations of fluctuating biological membranes
J Chem Phys. 132, 154107 (2010)
A. Pasqua, L. Maibaum, G. Oster, D.A. Fletcher
Biology under construction: in vitro reconstitution of cellular function
Nat. Rev. Mol. Cell Biol. 10, 644–650 (2009)
A.P. Liu and D.A. Fletcher
Membrane-induced bundling of actin filaments
Nat Phys. 4, 789–793 (2008)
A.P. Liu, D.L. Richmond, L. Maibaum, S. Pronk, P.L. Geissler, D.A. Fletcher
Curvature and torsion in growing actin networks
Phys Biol. 5, 026006 (2008)
J.W. Shaevitz, D.A. Fletcher
Load fluctuations drive actin network growth
Proc. Natl. Acad. Sci. U.S.A. 104, 15688–15692 (2007)
J.W. Shaevitz, D.A. Fletcher
Reversible stress softening of actin networks
Nature. 445, 295–298 (2007)
O.Chaudhuri, S.H. Parekh, and D.A. Fletcher
Actin polymerization serves as a membrane domain switch in model lipid bilayers
Biophys. J. 91, 4064–4070 (2006)
A.P. Liu and D.A.Fletcher
Fast, cheap, and somewhat in control
Genome Biol. 7, 114 (2006)
A.P. Arkin and D.A. Fletcher
Loading history determines velocity of actin network growth
Nat. Cell Biol. 7, 1219–1223 (2005)
S.H. Parekh, O. Chaudhuri, J. Theriot, and D.A. Fletcher
Photopatterning of actin filament structures
Nano Lett. 5, 625–628 (2005)
A.P. Liu, D.A.Fletcher
Compression forces generated by actin comet tails on lipid vesicles
Proc. Natl. Acad. Sci. U.S.A. 100, 6493–6498 (2003)
P.A. Giardini, D.A. Fletcher, J.A. Theriot